mad2l2 a4630 antibody Search Results


ki67  (Proteintech)
96
Proteintech ki67
AURKB knockdown suppresses cell proliferation, cell cycle progression, migration and invasion, and upregulates cellular senescence. A The RT-qPCR analyses detected shRNAs transfection efficiency in T24 and 5637 cells. B Cell viability was measured by the CCK-8 assay in T24 and 5637 cells. C The colony-forming assay detected the proliferation ability of a single tumor cell. D IF staining for <t>KI67</t> (red) and counter-staining nuclei with DAPI (blue) in T24 and 5637 cells. E Cell cycle analysis was measured by flow cytometry in T24 and 5637 cells. F Representative images of SA-β-gal staining for T24 and 5637 cells. G The wound healing assay demonstrated the capacity of migration in T24 and 5637 cells. H The transwell assay demonstrated the capacity of invasion in T24 and 5637 cells. (The magnification under the microscope is shown as marked in the figure. *p < 0.05, **p < 0.01)
Ki67, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mad2l2+a4630+antibody/KI67+Antibody/pmc10956193-80-7-10
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ki67 - by Bioz Stars, 2026-09
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AURKB knockdown suppresses cell proliferation, cell cycle progression, migration and invasion, and upregulates cellular senescence. A The RT-qPCR analyses detected shRNAs transfection efficiency in T24 and 5637 cells. B Cell viability was measured by the CCK-8 assay in T24 and 5637 cells. C The colony-forming assay detected the proliferation ability of a single tumor cell. D IF staining for KI67 (red) and counter-staining nuclei with DAPI (blue) in T24 and 5637 cells. E Cell cycle analysis was measured by flow cytometry in T24 and 5637 cells. F Representative images of SA-β-gal staining for T24 and 5637 cells. G The wound healing assay demonstrated the capacity of migration in T24 and 5637 cells. H The transwell assay demonstrated the capacity of invasion in T24 and 5637 cells. (The magnification under the microscope is shown as marked in the figure. *p < 0.05, **p < 0.01)

Journal: Journal of Translational Medicine

Article Title: AURKB promotes bladder cancer progression by deregulating the p53 DNA damage response pathway via MAD2L2

doi: 10.1186/s12967-024-05099-6

Figure Lengend Snippet: AURKB knockdown suppresses cell proliferation, cell cycle progression, migration and invasion, and upregulates cellular senescence. A The RT-qPCR analyses detected shRNAs transfection efficiency in T24 and 5637 cells. B Cell viability was measured by the CCK-8 assay in T24 and 5637 cells. C The colony-forming assay detected the proliferation ability of a single tumor cell. D IF staining for KI67 (red) and counter-staining nuclei with DAPI (blue) in T24 and 5637 cells. E Cell cycle analysis was measured by flow cytometry in T24 and 5637 cells. F Representative images of SA-β-gal staining for T24 and 5637 cells. G The wound healing assay demonstrated the capacity of migration in T24 and 5637 cells. H The transwell assay demonstrated the capacity of invasion in T24 and 5637 cells. (The magnification under the microscope is shown as marked in the figure. *p < 0.05, **p < 0.01)

Article Snippet: Antibodies used in IF were as follows: KI67 (1:200, 27309-1-AP, Proteintech, Wuhan, China), AURKB (1:50, A19539, ABclonal), MAD2L2 (1:50, A4630, ABclonal).

Techniques: Knockdown, Migration, Quantitative RT-PCR, Transfection, CCK-8 Assay, Staining, Cell Cycle Assay, Flow Cytometry, Wound Healing Assay, Transwell Assay, Microscopy

AURKB ablation upregulates the p53 pathway and suppresses BC cell progression via MAD2L2. A - B Cell growth was detected by CCK-8 assay ( A ) and colony-forming assay ( B ). C KI67 detection using IF staining. D Cell cycle analysis was measured by flow cytometry. E Representative images of SA-β-gal staining. F Western blot analysis of AURKB, MAD2L2, CyclinD1, p53, p21 and γH2A.X expression. G The wound healing assay demonstrated the capacity of migration. H The transwell assay demonstrated the capacity of invasion. (The magnification under the microscope is shown as marked in the figure. *p < 0.05, **p < 0.01)

Journal: Journal of Translational Medicine

Article Title: AURKB promotes bladder cancer progression by deregulating the p53 DNA damage response pathway via MAD2L2

doi: 10.1186/s12967-024-05099-6

Figure Lengend Snippet: AURKB ablation upregulates the p53 pathway and suppresses BC cell progression via MAD2L2. A - B Cell growth was detected by CCK-8 assay ( A ) and colony-forming assay ( B ). C KI67 detection using IF staining. D Cell cycle analysis was measured by flow cytometry. E Representative images of SA-β-gal staining. F Western blot analysis of AURKB, MAD2L2, CyclinD1, p53, p21 and γH2A.X expression. G The wound healing assay demonstrated the capacity of migration. H The transwell assay demonstrated the capacity of invasion. (The magnification under the microscope is shown as marked in the figure. *p < 0.05, **p < 0.01)

Article Snippet: Antibodies used in IF were as follows: KI67 (1:200, 27309-1-AP, Proteintech, Wuhan, China), AURKB (1:50, A19539, ABclonal), MAD2L2 (1:50, A4630, ABclonal).

Techniques: CCK-8 Assay, Staining, Cell Cycle Assay, Flow Cytometry, Western Blot, Expressing, Wound Healing Assay, Migration, Transwell Assay, Microscopy

Ablation of MAD2L2 suppresses BC cell progression via p53 DDR pathway. A , B Cell growth was detected by CCK-8 assay ( A ) and colony-forming assay ( B ). C KI67 detection using IF staining. D Cell cycle analysis was measured by flow cytometry. E Representative images of SA-β-gal staining. F Western blot analysis of MAD2L2, CyclinD1, p53, p21 and γH2A.X expression. G The wound healing assay for T24 and 5637 cells. H The transwell assay for T24 and 5637 cells. (The magnification under the microscope is shown as marked in the figure. *p < 0.05, **p < 0.01)

Journal: Journal of Translational Medicine

Article Title: AURKB promotes bladder cancer progression by deregulating the p53 DNA damage response pathway via MAD2L2

doi: 10.1186/s12967-024-05099-6

Figure Lengend Snippet: Ablation of MAD2L2 suppresses BC cell progression via p53 DDR pathway. A , B Cell growth was detected by CCK-8 assay ( A ) and colony-forming assay ( B ). C KI67 detection using IF staining. D Cell cycle analysis was measured by flow cytometry. E Representative images of SA-β-gal staining. F Western blot analysis of MAD2L2, CyclinD1, p53, p21 and γH2A.X expression. G The wound healing assay for T24 and 5637 cells. H The transwell assay for T24 and 5637 cells. (The magnification under the microscope is shown as marked in the figure. *p < 0.05, **p < 0.01)

Article Snippet: Antibodies used in IF were as follows: KI67 (1:200, 27309-1-AP, Proteintech, Wuhan, China), AURKB (1:50, A19539, ABclonal), MAD2L2 (1:50, A4630, ABclonal).

Techniques: CCK-8 Assay, Staining, Cell Cycle Assay, Flow Cytometry, Western Blot, Expressing, Wound Healing Assay, Transwell Assay, Microscopy

AURKB promotes BC growth and downregulats p53 DDR pahway by regulating MAD2L2 expression in vivo. A Images of dissected T24 xenograft tumors. B , C Tumor volume and weight in each group. D Representative images of IHC staining for MAD2L2, KI67, CyclinD1 and p53 in each group. E Western blot analysis of AURKB, MAD2L2, CyclinD1, p53, p21 and γH2A.X expression in each group (The magnification under the microscope is shown as marked in the figure. *p < 0.05, **p < 0.01)

Journal: Journal of Translational Medicine

Article Title: AURKB promotes bladder cancer progression by deregulating the p53 DNA damage response pathway via MAD2L2

doi: 10.1186/s12967-024-05099-6

Figure Lengend Snippet: AURKB promotes BC growth and downregulats p53 DDR pahway by regulating MAD2L2 expression in vivo. A Images of dissected T24 xenograft tumors. B , C Tumor volume and weight in each group. D Representative images of IHC staining for MAD2L2, KI67, CyclinD1 and p53 in each group. E Western blot analysis of AURKB, MAD2L2, CyclinD1, p53, p21 and γH2A.X expression in each group (The magnification under the microscope is shown as marked in the figure. *p < 0.05, **p < 0.01)

Article Snippet: Antibodies used in IF were as follows: KI67 (1:200, 27309-1-AP, Proteintech, Wuhan, China), AURKB (1:50, A19539, ABclonal), MAD2L2 (1:50, A4630, ABclonal).

Techniques: Expressing, In Vivo, Immunohistochemistry, Western Blot, Microscopy